Do bacteria grow on TSA plates? - sterile cotton
I am helping a teacher, some students have a science project. I have 10 (commercial preparation) triptych soy agar plates on their experiences. Show different surfaces around the school with a sterile cotton swab. For inoculated TSA plates, rubbed / directly rolled to plates buffer. After two days at room temperature (70 degrees F), I see the growth! What have I done wrong? How do I grow them?
PS - The floor bathroom, floor classroom, a wardrobe and desk.
Friday, January 22, 2010
Sterile Cotton Do Bacteria Grow On TSA Plates?
3 comments:
Hey, I'm a technician in biology at the university. provide students with the TSA plates with a bottle of sterile water. The swabs should be immersed in sterile water before swabing surface. Dip swab in sterile water to the surface of cotton, the Cotton Board. I just leave the dishes on the table again for a week at room temperature, then Soo odor. If you do not get growth, to say, a great effort to practice the very simple and have massive growth.
There was a sharp increase in the plates were. It would be better to moisten the swab with sterile saline solution, so that most of the pollutants accumulate on surfaces. If the bathroom on the floor with a disinfectant, you can not wipe a lot more growth. Take more time to think of the temperature for growth. There were signs that the test is moist (steam droplets inside the cover)? Try incubation of the plate in a warm, dark cupboard in my head for a few days.
If you have any unused plates, we could repeat the experiment. Or, as the panels are not open to students of May you touch the surface of the plate in the toes, agar-agar to kiss them sterile (!), Hair Scratches on the plate opening, put some money on the plate surface.
What it shows, you should enjoy the plates in a solution of diluted bleach for an hour or two before throwing the dishes! Hope this helps! If you are a local community college or university, you try to callsee the Biology Department, to see if they can contribute to the delivery of reports or otherwise, about this! Or see if you can make a trip to the university, college, or even organize the laboratory of the local hospital! Good luck in your germs Safari!
You can have a higher temperature than the bacteria grow best at around 45C.
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